Yunnan Paiyao in Established Fractures: Therapeutic Efficacy and Possible Mechanisms of Action in Rabbits
王天美 (Teen-Meei Wang); 張書源 (Shu-Yuan Chang);張成國 (Chung-Gwo Chang);蔡長海 (Chang-Hai Tsai);史中 (Chung Shih);王宜萱(Yi-Hsuan Wang)
對照組再分為假性左側腓骨切除(sham，S)組和左側腓骨切除 (fracture F)組。
1) F與F+Y組I但情中驗性磷酸?濃度都較S和S+Y組增加，同時F + Y組的濃度亦較F組為高。
另在術後90天，兩斷端間雖可見微量的橋形骨痂和聯合骨痂的形成，惟仍有相當距離的骨折間隙、(fracture gap)存在。F+Y 組在術後30天，可見所有(四種)骨痂的形成，其量隨時間的增加而增加。在術後60和90天,兩斷端間先被封閉骨痂和聯合骨痂充滿，後因進行重塑。
(rmodeling)步驟，使原先充滿骨痂的骨髓牌幾近相通。4) F+ Y組的鬆質骨骨量和單位面績總骨量都分別較F組多，惟其增加最隨時間增加而相對減緩。5) F+Y組的活性骨生成表面和骨沉積速率都 較F組多，且其骨生成速率較F組快1.4至1.7倍。 結論內服雲南白藥對骨折處有增加造骨細胞的數目和活性，加快各種骨痂和骨量的生成以及骨化的過程，從而促進骨折癒合。
Although Yunnan-Paiyao CY) has been shown stop bleeding, increase wound healing, increase blood circulation and degrade clots, recent study results have highlighted the therapeutic efficacy of Y in healing bone fractures. Methods.
In our study, six rabbits were used in each group, the microradiographic and histomorphometric changes of the fibulae group between 0 mm- and 6 mm-operatedfracture (F) groups with and without oral treatment of 0.25g Y per day for30, 60 or 90 consecutive days were evaluated.
Serum levels of calcium, phosphorous and alkaline phosphatase (ALP) activities were also measured. Results. Increases in body weight, trabecular bone volume, total bone volume are, active bone formation surface, bone apposition rate and bone formation rate of the fracture in situ were observed at all times in the fibular fractures treated with Y (F+ Y) rabbits. All four kinds of callus, such as anchorage callus, sealing callus, bridging callus, and uniting callus were formed at 30-days in the- F+ Y group and significantly increased, at 60- and 90-days in the F+ Y groups. The progress of remodeling procedures of fracture healing was remarkable at 90-days in the -F+ Y group.
However, no union was monetary time in all F groups. Additionally, serum concentrations of ALP were significantly increased at 30,60- and 90-days in both F+Y and F groups when compared with the control group (S) and S treated with Y(s+ Y). However, serum concentrations of ALP were slightly, but not significantly higher, except for the group at 90-days, in the F+ Y group than in the F group.
These results indicate that callus formation and cancellus bone formation developed in the F+ Y group and that shortening of the inflame F+Y stage and promotion of reparative _and remodeling stages in the F+ Y group coincided with the maximal increase in bone formation.
Conclusions. Fracture healing of the rabbit fibulae was enhanced rapidly after using Y orally. Its possible mechanisms might be explained that Y caused and then increase the number and activity level of osteoblasts, thus, callus formation occurred in the fractured site, and induced bone formation. The method of operated fibular fracture.